Kim's Research Interests

 

 

Dual-color imaging for isolating olfactory bulb output streams in mice


It is known that different brain areas can process segregated aspects of the sensory space as for example the “what & where” pathway in vision. However, whether different regions in the olfactory cortex receive different functional input from mitral and tufted cells (MTCs) remains unknown. MTCs, the output neurons of the olfactory bulb, transmit sensory information to the olfactory cortex. Within the olfactory cortex, MTCs project to several areas including the anterior olfactory nucleus, piriform cortex, olfactory tubercle, cortical amygdala and the lateral entorhinal cortex.

In my master thesis we started to ask whether these anatomically distributed pathways enable parallel processing in olfaction. Using stereotactic injections and retrograde tracing by means of AAV constructs encoding for red and green calcium indicators (GCaMP6 and jRCaMP1a), we label different olfactory bulb output streams based on their axonal target in the olfactory cortex. Performing widefield and high-resolution two-photon microscopy in the olfactory bulb we compare the physiological features of anatomically defined olfactory bulb streams. Using this approach we want to investigate if segregated aspects of the sensory space are processed in parallel streams in the olfactory system.

Keywords: olfaction • two-photon microscopy • calcium imaging • genetically encoded calcium indicators • stereotactic injection • retrograde tracing

 

 

Team